En säker bakteriediagnostik kräver en korrekt provtagningsteknik, ett lämpligt transportsätt och rätt odlingsteknik. På grund av centralisering av verksamheter och ekonomiska begräsningar, är transport av prover till mikrobiologilaboratorium mycket vanligt. Detta medför behov av ett transportmedium som håller känsliga bakterier vid liv under långa transport- och förvaringstider. Dessutom skall transportrören klara av maskinell utodling som kräver ett vätskebaserat provtagningsmedium. Denna övergång minskar även arbetsbelastning och förbättra ergonomin hos personalen.

Syftet med denna studie var att undersöka den antibakteriella effekten hos ett polyestertyg med tunna invävda koppartrådar. Frågeställningen som skulle besvaras var ifall koppartyget hade en baktericid eller bakteristatisk effekt. Koppartyget är ännu i prototypstadie och om det visar sig ha antibakteriella egenskaper är det tänkt att användas inom klinisk verksamhet för att förhindra bakterietillväxt i sår och andra känsliga lokaler. Koppar är ett essentiellt spårämne, men har också antimikrobiella egenskaper som utövas genom ett brett spektra av mekanismer där skador på cellmembranet är en av de viktigare. Metoderna som användes för att inokulera bakterier på tyget var absorptionsmetoden, där en näringsbuljong innehållande Staphylococcus aureus ATCC 6538 pipetterades på tygproverna, och transfermetoden där tygproverna trycktes mot en agar som racklats med peptonsaltlösning innehållande S.

I denna rapport predikteras livslängden, med avseende på utmattning, för en hjullastarskopa. Två av de mest kritiska svetsarna utvärderas enligt IIW:s (International Institute of Welding) rekommendationer genom nominella spänningsmetoden, hot spot-metoden och effective notch-metoden. Beräkningarna utförs med hjälp av finita elementmetoden. Genom att utföra töjningsmätningar på hjullastarskopan i drift erhålls spänningsdiagram. Dessa uppmätta spänningsdiagram analyseras med rain-flow count-metoden.

The objective of this work was to evaluate and compare three field methods for detection of mastitis, California Mastits Test (CMT), DeLaval cell counter DCC (DCC) and FMA2001 Farm Milk Analyzer (FMA2001). Furthermore, to investigate at what cell count level clinical symptoms of mastitis are seen, and finally to check if the clinical findings and cell counts could be related to bacteriological findings. DCC and FMA2001 are new analytic instruments to be used on dairy farms for detection of mastitis. CMT has been a widely used cow-side-test of milk quality in mastitis control efforts, but it cannot be used for estimating cell counts in scientific studies or investigations. Two hundred and eight quarter milk samples from 52 cows were analysed with DCC and CMT, and 168 of these samples with FMA2001. Microbiological examination was done on 38 milk samples with CMT scores of 4 and 5.

 Conclusion:Medical treatment may have a role in platelet count after transfusion. Since the TACSI platelets passed the quality requirements, and the vast majority of patients platelet count increased after TACSI platelet transfusion, the TACSI platelets will replace the old method to produce platelets at the Uppsala University hospital.  Methods: A new approach that pools 8 buffy coats (TACSI platelets) that were separated into 2 units instead of 4-6 buffy coats pooled to 1 unit was investigated in this study. After the platelets were extracted from the buffy coats their quality was controlled and subsequently the platelet product was evaluated in 96 patients. Results: The results showed that 80 % of the platelet units passed the European quality requirements. Further, the platelet count was increased in most patients that received TACSI platelets. Conclusion: Medical treatment may have a role in platelet count after transfusion.

Two haematological instruments intended for small animal clinic use were evaluated at the Department of Clinical Chemistry, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden. The QBC-V and Vet abc were compared to a reference instrument, the Cell-Dyn 3500. Fresh blood samples were obtained from canine and feline patients from the University Veterinary Hospital, SLU. The QBC-V was used for 111 canine samples and 78 canine blood samples were analysed with Vet abc. From cats, 23 samples were analysed with both instruments.

The purpose of the present study was to investigate the cow-level association between acute hoof lesions and the somatic milk cell count in dairy cattle. Cows from three loose-housed Swedish dairy herds were enrolled in the study if they had an acute hoof lesion treated by a veterinarian or professional hoof trimmer during the preceding year, according to farmer records. Studied lesions were sole ulcer, dermatitis, interdigital necrobacillosis and hoof abscess, in each case causing lameness. For each hoof-diseased cow, 0 to 3 healthy cows were included, matched with respect to herd, breed, parity and lactation stage. Cell counts were obtained from monthly test recordings from one month before to three months after the hoof-lesion diagnosis, and log-transformed.

Hematology-instruments that analyze blood cells can at specific cases be followed by manual counting with a hemocytometer. The plastic hemocytometer which has a fixed coverslip and is assigned for disposable use, appears to reduce the problems arising from the use of the traditional glass hemocytometer, for instance coverslip-application difficulty and dust particles. Capillary whole blood sampling may be useful for patients at the emergency department and in addition, a smaller volume of blood is taken compared to venous blood sampling. This study will focus on the platelet- and leukocyte count (PLT and WBC) in venous and capillary blood, in order to compare the plastic- and glass hemocytometers with Sysmex XN-2000 (Sysmex Corporation, Kobe, Japan), and verify if capillary whole blood sampling can be used for the measurement of these hematological parameters. 30 subjects donated both capillary- and venous blood samples and these blood samples were analyzed with glass- and plastic hemocytometers with Sysmex XN-2000 as the reference method.

Denna uppsats handlar om tektologi, en systemteori utvecklad av en rysk vetenskapsman vid namn Alexander Bogdanov mellan åren 1912 och 1928. Vi har i uppsatsen haft för avsikt att utreda om tektologin har något att tillföra modernt systemtänkande. Detta har vi gjort genom att låta Stafford Beers viable system model representera det moderna systemtänkandet, och därefter jämföra en modellering av en organisation med VSM, med en modellering av samma organisation med en egenutvecklad modell baserad på tektologi. Resultaten visar att tektologin har en hel del att tillföra modernt systemtänkande. Framför allt ger den nya infallsvinklar till modelleringen och belyser delar av organisationen som man inte kan se med VSM.

This thesis aims to identify the building integrated photovoltaic solutions available in the market with respect to general pros and cons of different systems and a technical description of there attachment system. Investigate and provide examples of various building integrated photovoltaic solutions in Sweden and describe the experiences and results of these projects.The methods used to obtain relevant information are interviews with individuals involved in significant projects and a thorough search of different solutions from solar suppliers.The conclusion is that solar energy is needed for a sustainable society. There are endless opportunities regarding BIPV, with many companies with different products and individual solutions. But so far it?s not so customary in Sweden.

Brämhults Juice AB is one of Sweden?s leading producers of fresh fruit juices and drinks. The fruit drinks last 3 days once opened and when stored in a refrigerator kept at 7°C. Since factors that shorten the drinks? best before date are usually not noticed until the entire batch has expired, it would be advantageous to find a way to accelerate the drinks? natural deterioration, so that an unusal short best before date is noticed before more products are shipped off to the stores.

The Annexin-V/propidium iodide (AV/PI) assay detects early membrane changes in spermatozoa and viability using flow cytometry. It is an objective method that evaluates a much larger number of single spermatozoa compared with the usual estimation of motility, the most commonly used method for routine sperm evaluation. In the present study four ejaculates from four stallions were used in a split-sample design to investigate whether and how the number of intact spermatozoa is affected over time and after incubation at different temperatures, either those recorded during shipment of stallion semen for AI (9 or 5°C) or room temperature (20°C). The Annexin-V results did not reflect the motility results that were monitored in parallel. While motility decreased over time, the number of spermatozoa with intact membranes (viable) remained unchanged for at least 21 hours following a short period of initial instability.

Brämhults Juice AB is one of Sweden?s leading producers of fresh fruit juices and drinks. The fruit drinks last 3 days once opened and when stored in a refrigerator kept at 7°C. Since factors that shorten the drinks? best before date are usually not noticed until the entire batch has expired, it would be advantageous to find a way to accelerate the drinks? natural deterioration, so that an unusal short best before date is noticed before more products are shipped off to the stores.

Energy efficiency is important both to reduce costs and to reduce greenhouse gas emissions in the atmosphere. Reducing costs will also help maintain business competitiveness.Scania in Södertälje is a company where the optimization of energy use is an ongoing and continual process. One area with potential to make energy and cost savings is the effectiveness of the ventilation systems in offices and industrial premises at Scania. During the summer months an increased demand for cooling occurs, leading to increased ventilation and a peak in district cooling system usage. Sorptive cooling is a technology where the supplied air is cooled by applying external heat.

With an increasing production of biogas follows an increased amount of digestate. This could potentially be a valuable source of fertiliser with the destruction of weed seeds as a possible advantage. However, there have been some concerns regarding the product still containing viable seeds of weed. In this Bsc thesis the results of previous research on the fate of weed seeds in biogas production is summarized. It is also a comparison of research on the effect of similar processes such as silage, compost or animal passage on seeds. The purpose of this is to evaluate risks of spreading viable seeds, potentially problematic species and perhaps to find out how to eliminate the risks. The literature review revealed that previous research mainly indicates low risks regarding many of the tested weed species, but not all studies show the same result, therefore extended research on the subject is desirable.